- Strain isolation and characterization
- We isolate novel syngas utilizing strains and characterize the isolated strains (growth factors, metabolites, biochemical and physiological properties etc.).
- Transcriptomic analysis
- We also analyze transcriptome profiles for understanding metabolic features of acetogens. Based on the results, acetogens can be genetically engineered to improve the production of biofuels
- Genetic/Metabolic engineering
- In this part, our missions are;
1)Development of genetic tools to engineer acetogens for better CO-utilization and biochemical production.
2)Development of non-replicating/ replicating shuttle vectors for E. coli/ Acetogens.
3)Construction of novel metabolic pathways for valuable biochemicals and biofuels (butanol, ethanol, 2,3-butanediol, etc)
- Our research team is trying to establish the syngas fermentation process for hyper chemical production. First, we design high efficiency gas delivery device to generate micro bubble. Second, the optimized process design is required to increase biocatalyst concentration. Also, we develop mathematical models to quantify physiological characteristics of syngas fermenting microbes and simulate process operational parameters such as chemical concentration, gas consumption and mass transfer rate. Based on the process simulation, techno-economic analysis is conducted.
- Effective redox bioelectrocatalysts are the crux to design and development of the next generation of bioelectrochemical systems (BESs) such as microscale electrochemical biosensors, biomedical devices, and biofuel cells. For advanced BESs, the enzymes should have high catalytic activity, stable, and be inexpensive. Also, bioelectrocatalysis process necessitates specific methods for mediation and enzyme immobilization to optimize electron transfer (ET) kinetics between the enzyme and electrode. Finally, electrode fabrication that allows operation close to the theoretical redox potential of the enzyme will maximize the potential difference between anode and cathode and hence maximize the output of the electrochemical communication efficiency.
- A microbial electrochemical system (MES) is a system that uses microorganisms as biocatalysts to produce chemicals or energy. The factors involved in the catalytic reaction of microorganisms in MES are more diverse and complex than chemical reactions, so research on MES requires a broad background; such as extracellular electron transfer (EET) system of electrochemically active bacteria, structural characteristics of the conductive biofilm on the electrodes, and relationship between EET system and the biofilm configuration. This background lead to interspecies electron transfer in methanogens and EAB mixed culture systems in the methane production process.
- We have extracted DNA from bioreactors and aerosol samples to study the microbial composition and the functional genes of the sampled community. PCR-based sequencing was used for rapid microbial community analysis while shotgun metagenomics sequencing was used to characterize the microbial and functional composition of the sampled community. For e.g. in aerosol samples, we studied the microbial composition changes in between the non-event day (good PM2.5 condition) and event day (bad PM2.5 condition). Also, by using the bioinformatics approach, we can identify the functional genes presents in the sampled community.